Российская академия наук


INVESTIGATION OF INTENSITY OF NITRIC OXIDE PRODUCTION IN RAT BRAIN AFTER ISCHEMIC STROKE BY METHOD OF EPR SPECTROSCOPY



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INVESTIGATION OF INTENSITY OF NITRIC OXIDE PRODUCTION IN RAT BRAIN AFTER ISCHEMIC STROKE BY METHOD OF EPR SPECTROSCOPY

Gainutdinov Kh.L. 1,2, Andrianov V.V. 1,2, Yafarova G.G. 1,2, Iyudin V.S.2, Denisov A.A. 3, Pashkevich S.G. 3, Khotyanovich M.O. 3, Kulchitchkii V.A. 3

1Kazan Federal University, Kazan, Russia; 2Kazan Physical-Technical Institute of Russian Acad. of Sci., Kazan, Russia; 3Institute of Physiology of Nat. Acad. of Sci. of Belarus, Minsk, Belarus; kh_gainutdinov@mail.ru
In present time the development of brain ischemia and subsequent occurrence of stroke associated with disorders of cerebral blood flow, as well as with the disrupting in the regulation of the system of nitric oxide (NO). Some authors consider activation of the system NO as one of the pathogenic factors in the development of stroke, and the other, on the contrary, indicate of its tread role. System NO is one of the most studied systems of the organism. In our work it was conducted research of the role of NO in the synaptic conductivity during modeling of hemorrhagic and ischemic strokes with the use of method of electron paramagnetic resonance (EPR). It was studied the content of NO in the tissues of the brain (the hippocampus), the heart and the liver of rats by method of EPR spectroscopy using the methods of spin traps. As a spin traps applied a complex of Fe2+ with diethylditiocarbamate (DETC) - (DETC)2-Fe2+-NO. This complex is characterized by a distinctive range of EPR with the value of the g-factor g=2.035 and the triplet super thin structure. Registration of prepared samples was carried out at the spectrometers of the EPR EMX/plus X-band of the company "Bruker" and spectrometer ER 200E SRC of the company "Bruker" in the X range (9.50 GHz) with the modulation of the magnetic field of 100 kHz, amplitude modulation 2Gs, power of microwave radiation of 30 mW and with a time constant of 200 ms, also earlier was carried out verification of the registration of the EPR spectrum of exogenous donor nitric oxide - nitroprousside sodium.

We found that the level of production NO through three days after a hemorrhagic, and after an ischemic stroke in the hippocampus is decreased in several times and it should be noted that a fundamental difference in the two experimental series was not observed. It was also found that the decrease in production of NO in the tissues of the heart and the liver occurs to a greater extent.



This work is supported by RFBR, grant nr. 12-04-97033_Bel.

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