Российская академия наук


Activity of alcohol dehydrogenase at different levels of alcohol motivation



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Activity of alcohol dehydrogenase at different levels of alcohol motivation

Mescheryakova M.Yu., Saley A.P., Vashanov G.A., Lavrinenko I.A.

Russia, Voronezh State University, saley64@yandex.ru


The studies were conducted on non-linear rats weighing 200-250 g, kept in individual cages in the same conditions of feeding, at the VSU, department of human physiology and animals. All manipulations with the animals were carried out in accordance with the rules of humane treatment of laboratory animals and the guidelines MoH.

All rats were tested for susceptibility to the consumption of ethanol by the method of free choice. 3 different groups of animal were distinguished: which have consumed to 15 % of ethanol (water was more preferred than ethanol) or 50 % of ethanol («intermediate») or more than 50 % of ethanol (ethanol was more preferred than water). Of the latter were formed four groups of animals to 8 individuals. In rats the first and second groups which was consisted of 4 female and 4 male individuals respectively. The formation of alcohol motivation was tested by the method of «forced consumption» 15 % ethanol on the latter group.

The rats of the third and fourth groups, which also were consisted of 4 female and 4 male individuals, were tested by the method of «free choice» with free access to water and 15 % ethanol. Fluid intake was recorded by using graduation lines of drinking-bowl and activity of alcohol dehydrogenase was measured by using a spectro-

photometer UV-2550 SHIMADZU by reducing of the optical density at 340 nm. Daily water consumption of intact rats was 11,61,03 ml/100 g mass; activity of ADH in plasma and liver homogenates of male rats was 0,810,041 and 1,220,072 nmol / min * ml, respectively, while among female rats this value was 23 % (1,050,072 and 1,590,104 nmol / min * ml, respectively).

Against the background of the formation of alcohol motivation in rats ethanol consumption of the first and second groups were recorded on 18, 45 and 90 days. For the period from 45 to 90 days alcohol addiction increased by 9.5 % and 15 % compared to 18 day consumption of ethanol, respectively.

Activity of ADH in plasma and rat liver homogenate at 45 and 90 days also increased after the start of alcohol consumption compared to intact animals. However, in plasma and liver of male rats on 18th day of their alcoholism, changes in the activity of ADH were not found compared with intact animals. Rats of the third and fourth groups, which had a «free choice», consumed more liquid than rats, which was forced to consume ethanol. However, during the three month of forming alcohol motivation, rat’s consumption of ethanol increased and consumption of water reduced.

Studies have shown that during the formation of alcohol motivation in rats, ethanol consumption is directly proportional to the activity of ADH in plasma and liver. Adaptation to ethanol is faster in female rats than in male rats.


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