1 Establishment of the Russian Academy of Sciences Institute of chemical physics of N. N. Semenov of the Russian Academy of Sciences, Moscow, Russia; 2 Federal public budgetary educational institution of additional professional education institute of professional development of FMBA of Russia, Moscow, Russia; 3 Clinical hospital № 123 of FMBA of Russia, Moscow, Russia
An important task of neuroscience is the development of representative markers of brain tissue preservation. Dysfunction of cerebral blood flow that results from obliteration of the cerebral arteries, reducing cerebral tissue perfusion and following this process a sharp decline in consumption of oxygen and glucose is the basis of all neurotrophic CNS dysfunction. Energy shortages and lactic acidosis are pathobiochemical triggers of cascade reactions in all CNS cell pools and leading to the formation of cerebral infarction by two basic mechanisms: necrosis and apoptosis.
A promising method of biochemical and neuromapping of the brain in the evaluation of cerebral energy metabolism is a non-invasive method for detecting and analyzing the DC-potential (mV), of brain reflecting of the blood-brain barrier potentials.
Measurement of DC-potential allows us to estimate the local brain damage and the extent of brain changes.
A results of numerous studies suggest DC-potential related with cerebral energy metabolism indices: the consumption of oxygen and glucose, glycogen storage, and the concentration of ATP and other energy metabolites, as well as the ratio of oxidized and reduced forms of respiratory enzyme NAD-NADH and the intensity of free radical reactions of lipid membranes brain.
In our study, we investigated the DC-potential patients with ischemic brain damage of various locations. It was revealed that the focus of ischemic stroke is three-fold decrease of DC-potential compared to the area of the brain that is free of ischemia. This reduction in DC-potential shows a corresponding reduction in cerebral energy metabolism caused by reduced cerebral blood flow, a decrease of membrane potentials BBB, increased permeability of the capillaries and the fall in glucose uptake in the lesion.
Thus, the energy metabolism of the brain investigated by the method of non-invasive registration of DC-potential, is a measure of the degree of brain damage and the degree of preservation of brain tissue with neurotrophic dysfunction.